FastQCFastQC Report
Mon 26 Dec 2016
H2WLMBGX2_n01_25_125_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2WLMBGX2_n01_25_125_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16342066
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCCTA30100.017.7538268
ACCTAGG44900.016.81586510
CCTATTA32800.016.60797311
TACCTAG49000.015.9717519
TTACGGG13850.015.682588
ATACCTA50900.015.5788998
CTATAAG36300.015.49339946
CGCTATG31900.015.47205629
TAGGTAC50350.015.201282513
GTCGCTA32900.014.78879627
GTGGATA51750.014.724954
TATAAGC38700.014.7089947
TAAGTCG27850.013.8648629
CGTAACG22200.013.83989742
CGGGACT22350.013.7349771
CGCGAAT27400.013.7211791
ATGCGTG31300.013.6599546
GGATACC59950.013.5735896
CATGCGT30400.013.3836635
CGGACAT51900.013.0257931
Produced by FastQC (version 0.11.2)