FastQCFastQC Report
Mon 26 Dec 2016
H2WLMBGX2_n01_25_125_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2WLMBGX2_n01_25_125_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16418134
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTAGG39050.019.33566910
TACCTAG44700.017.585969
ATACCTA44900.017.4309448
TAGGTAC44950.017.2581513
TTACGGG13650.015.9128988
GTGGATA52150.014.6131164
CCTAGGT54200.014.37628211
GGATACC54750.014.3599516
GTCGCTA29750.014.26569727
GTACCCA54100.013.89344316
TAAGTCG24250.013.3645669
CGCTATG31300.013.0114329
CTAGGTA59800.012.97235212
CGCGAAT23750.012.6362431
GTCCCTA23500.012.6174618
ACATGCG33550.012.5392684
CTACACG24800.012.51204412
GATACCT62800.012.3528047
AGTTACG31050.012.216126
CCTATTA25800.012.16071511
Produced by FastQC (version 0.11.2)