FastQCFastQC Report
Mon 26 Dec 2016
H2WLMBGX2_n01_25_0625_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2WLMBGX2_n01_25_0625_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13485142
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTAGG22200.013.04538410
CTACACG20900.012.86709512
ATACCTA25700.012.4761918
CGCGTAT14300.012.0613651
TACCTAG25250.011.8792349
CGCGAAT15900.011.7154541
TCCCTAC27000.011.2369769
CCGGGAT56150.010.9353511
CCTAGGT27600.010.49310411
CCCTACA29000.010.34312610
CGGGCAT31750.010.3214891
TAGGTAC26950.010.10649313
GGAAAAT71950.09.924351
GCGAAAT36750.09.7619931
GCGGAAT17800.09.6897491
CTAGGTA31900.09.40287612
TTCCCTA30000.09.1939238
TAAGTCG12850.09.1223429
TTACGGG8701.0913936E-119.1146678
CCGGAAT32650.09.0861051
Produced by FastQC (version 0.11.2)