FastQCFastQC Report
Mon 26 Dec 2016
H2WLMBGX2_n01_25_0625_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2WLMBGX2_n01_25_0625_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16969863
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAT21300.013.92828751
CGGGCAT36400.010.6144021
GTCCCTA21300.010.3599848
GCGAATC35100.010.2182042
CGGGGAT56750.010.1514821
CCGGGAT68100.09.7766281
GGAAAAT101150.09.6857071
GGCGAAT22550.09.6376881
CGCGATA15850.09.57641
CGATAGT18150.09.3098123
GCGGAAT24500.08.8706071
CGAATCC55350.08.8469213
GTCGAAT29900.08.6530441
TAAGCGG16750.08.64645420
CCCGACT33350.08.5854111
TTAGCGT31800.08.4571918
CTCGAAT30200.08.4528591
GGAGAAT70150.08.3598811
CTCGTAT19000.08.3518581
GGAAAAC64050.08.3481731
Produced by FastQC (version 0.11.2)