FastQCFastQC Report
Mon 26 Dec 2016
H2WLMBGX2_n01_25_0625_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2WLMBGX2_n01_25_0625_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14343577
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTAGG34350.018.3680310
TACCTAG36250.017.8809099
TAGGTAC36350.017.26292213
ATACCTA38950.017.172648
CTACACG23050.015.25720912
CTATAAG30900.015.07389746
CCCTACA29700.013.93038410
CCTAGGT44650.013.89937811
GTACCCA46600.013.39211316
GTGGATA47150.013.3106454
TATAAGC34850.013.26480147
GTCCCTA24700.013.2607928
CCTATTA25350.013.19283411
GTCCTAT34600.013.16793843
GGATACC51750.013.0603156
CTAGGTA49400.012.77232612
TCCCTAC29300.012.7085489
CCTATAA38350.012.50672645
CCGGGAT53000.012.3671291
CGGGACT20150.012.3267511
Produced by FastQC (version 0.11.2)