FastQCFastQC Report
Mon 26 Dec 2016
H2WLMBGX2_n01_125_50_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2WLMBGX2_n01_125_50_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23470041
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAT30150.014.3017991
ACCTAGG41150.010.89215610
CCGGGAT101400.010.81825351
CGGGGAT85900.010.3608291
CGGGCAT54700.010.1532851
ATACCTA46800.010.0192298
GTCGGTT33100.09.6922141
TACCTAG48300.09.4938849
CGCGATA19800.09.4050172
GCGAATC50450.09.0912062
CTCGGAT53950.09.015621
GCCGAAT39100.08.998941
TTAGCGT44500.08.9875068
GGGAGAT113700.08.98047351
GGGAAAT78600.08.9092571
GCCAGAT105100.08.8947671
CCTAGGT49400.08.86394911
TAGCGTC46400.08.8423639
GGAAAAT128650.08.7144811
TAGGTAC52350.08.49615913
Produced by FastQC (version 0.11.2)