FastQCFastQC Report
Mon 26 Dec 2016
H2WLMBGX2_n01_125_50_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2WLMBGX2_n01_125_50_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16726464
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTAGG26500.014.44137610
CTACACG33500.012.24747612
CCTAGGT30300.012.17535711
TCCCTAC33550.011.8178039
ATACCTA34300.011.6599138
TTCCCTA38850.011.359278
GCCGAAT27850.011.1469291
CCCTACA40450.010.90995310
TAGGTAC35350.010.82618413
TACCTAG34450.010.8085159
GGAAAAT89950.010.162081
CCGGGAT70700.010.0016671
CTAGGTA39500.09.688807512
GCGAAAT43250.09.5704651
CCTACAC52100.09.33086611
ACACTCT44300.08.8764451
GGGAAAT54650.08.7732921
GGATACC45900.08.56415756
CGGGGAT56800.08.5626591
GCGGAAT23100.08.5113961
Produced by FastQC (version 0.11.2)