FastQCFastQC Report
Mon 26 Dec 2016
H2WLMBGX2_n01_125_50_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2WLMBGX2_n01_125_50_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15263998
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTAGG32550.015.88856310
ATACCTA38000.015.0615218
TACCTAG38400.014.2761219
CGCGTAT25800.014.0392221
TAGGTAC41500.013.04380913
CCTAGGT40350.012.9028711
TAAGTCG21900.012.7522059
GCGTATT26900.012.0507822
CGCGATA22700.011.5494461
CTAGGTA48000.011.13376212
GATACCT51200.010.7744327
CGCGAAT17650.010.7495791
GTCGCTA26700.010.72601827
CGCTATG26750.010.70881329
GGATACC51900.010.4977966
GTGGATA50650.010.3483164
CTATAAG27700.010.33801746
CCGGGAT55050.010.2768491
TCCCTAC27700.010.2065389
GTCCCTA24500.010.1323638
Produced by FastQC (version 0.11.2)