FastQCFastQC Report
Mon 26 Dec 2016
H2WLMBGX2_n01_125_25_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2WLMBGX2_n01_125_25_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13253836
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTAGG28950.016.67277710
TAGGTAC33100.014.582602513
ATACCTA35250.014.4755258
CGCGATA19850.014.4244211
TACCTAG36700.014.0913839
TCCCTAC26150.013.9753489
CTACACG22050.013.75965212
TAAGTCG18750.013.6068929
CGGGACT17750.013.6044351
CCCTACA26800.012.73587710
TTACGGG9750.012.7300368
TACACGA26350.012.43019713
GGATACC42050.012.2187676
CGCGAAT18150.012.1642181
CTATAAG23300.012.14276146
CCTAGGT40000.012.06710411
GTGGATA39500.012.0472374
CGCTATG21500.011.87938129
CGCGTAT23550.011.8651971
GTCCCTA18750.011.5843328
Produced by FastQC (version 0.11.2)