FastQCFastQC Report
Mon 26 Dec 2016
H2WLMBGX2_n01_125_25_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2WLMBGX2_n01_125_25_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9959556
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTAGG25450.020.1856310
ATACCTA31000.017.5729068
TAGGTAC30250.017.21100813
TACCTAG30500.017.1825499
GTGGATA30100.016.8400214
CTACACG14500.016.64495512
CTATAAG21750.016.02244446
TTACGGG8500.015.8195428
CATGCGT19450.015.4237255
ATGCGTG19900.015.24836
CGCGAAT17500.014.9799061
ACATGCG20750.014.9560254
TAAGTCG20800.014.7526669
GTACCCA36250.014.6477816
GGATACC35850.014.5237786
CCCTACA21400.014.50015410
CGGAATA16300.014.39305968
CGGGACT15150.014.343711
TATAAGC24850.014.29935447
CGCTATG20100.014.0801329
Produced by FastQC (version 0.11.2)