FastQCFastQC Report
Mon 26 Dec 2016
H2WLMBGX2_n01_125_125_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2WLMBGX2_n01_125_125_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8486941
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTAGG19150.018.9041810
ATACCTA23300.016.8689828
TACCTAG22350.016.8146929
TAGGTAC21850.016.72608613
GTCCCTA14400.016.5206288
CTATAAG17450.015.22483446
GTGGATA24000.015.0862734
CGCGAAT12900.014.9744421
ATGCGTG17800.014.3356756
TATAAGC19900.013.6955747
CGGGACT11350.013.6763121
CCTATTA16400.013.45468311
CATGCGT18650.013.3124375
GTACCCA27500.013.2899516
ACATGCG16900.013.2626574
ACCTATA16900.013.0586946
CTACACG11450.012.94800612
TTACGGG6950.012.8981818
GTCCTAT21700.012.8834243
TGCGAGT16600.012.46179311
Produced by FastQC (version 0.11.2)