FastQCFastQC Report
Mon 26 Dec 2016
H2WLMBGX2_n01_125_125_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2WLMBGX2_n01_125_125_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10155108
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTAGG25550.017.1378310
TTACGGG8550.016.9366028
CTACACG15700.016.25105512
ATACCTA28600.015.5513018
TAGGTAC28000.015.51528813
TACCTAG29250.015.3235919
GTGGATA31100.013.9706064
CTATAAG21300.013.60690446
CGCTATG18300.013.57931929
TATAAGC22450.013.36931247
AGTTACG19100.012.9988936
CCCTACA21400.012.88900110
CGGGACT13750.012.7952261
TAAGTCG14600.012.7521669
CCTAGGT34800.012.68171711
GTCGCTA20000.012.59466927
ACATGCG18900.012.5890324
CGCGAAT13600.012.1753881
GTACCCA35750.012.15189416
GGATACC37300.012.1107136
Produced by FastQC (version 0.11.2)