FastQCFastQC Report
Mon 26 Dec 2016
H2WLMBGX2_n01_125_125_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2WLMBGX2_n01_125_125_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10402082
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAAG26000.020.56892246
CCTATTA23400.019.15536511
GTCCCTA23550.019.0331738
TATAAGC28550.018.72924847
CGCGAAT19050.018.65161
TTACGGG12450.018.2781968
CATGCGT20800.018.0706655
CGGGACT14600.017.9570331
ATGCGTG22600.017.6996526
ACCTAGG28950.017.62665410
ACATCCG32100.017.08996455
ACATGCG22150.016.5022474
GCCAGTA27850.016.3501821
CTACACG14250.016.2114112
CTATTAA27800.016.12350312
CCCTACA17650.016.01864610
GTGGATA32800.015.9801444
TAGGTAC32850.015.63887413
TAAGTCG24050.015.4833659
TACCTAG33450.015.358439
Produced by FastQC (version 0.11.2)