FastQCFastQC Report
Mon 26 Dec 2016
H2WLMBGX2_n01_125_0625_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2WLMBGX2_n01_125_0625_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11140916
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTAGG22700.015.79646610
ATACCTA25650.014.7862418
TTACGGG8200.013.8756258
TACCTAG27350.013.7411049
CGCGTAT17350.013.3218241
CGCGAAT16100.013.2847741
TAGGTAC27500.013.16487913
CTATAAG18800.013.02985646
ATGCGTG20700.012.9938876
TAAGTCG15800.012.8750069
TATAAGC21200.012.52988647
CGGGACT16000.012.0741461
CATGCGT20100.012.0092215
TACGGGG12400.011.9563669
GTCCCTA16450.011.947078
CTACACG17150.011.86178712
TTAGCGT21850.011.8348378
ACATGCG21800.011.705664
AGTTACG17300.011.5610356
CCTAGGT31550.011.47498611
Produced by FastQC (version 0.11.2)