FastQCFastQC Report
Mon 26 Dec 2016
H2WLMBGX2_n01_125_0625_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2WLMBGX2_n01_125_0625_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12036836
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAT15950.015.5705021
TAAGTCG18000.015.5138499
ACCTAGG25300.014.03542310
CTACACG16800.013.749330512
CGGGACT18750.013.6132331
ATACCTA26450.013.4251898
TACCTAG27500.013.0379029
CGCGTAT23700.012.8075221
CGCGATA20650.012.026611
ATAAGCG18100.012.00085719
TAGGTAC28550.011.95472913
ATGCGTG24100.011.8749856
TTACGGG9650.011.7895038
CTATAAG25600.011.72646646
TAAGCGG18800.011.55449820
CCCTACA25900.011.44741710
TAGTCAT27750.011.4313316
CATGCGT22450.011.365285
TCCCTAC24400.011.303359
GTCGCTA22700.010.9450127
Produced by FastQC (version 0.11.2)