FastQCFastQC Report
Mon 26 Dec 2016
H2WLMBGX2_n01_125_0625_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2WLMBGX2_n01_125_0625_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13294174
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGGG10900.017.7141748
CGGGACT20050.016.8611341
GTCCCTA20750.015.9519048
TAAGTCG21150.015.8131769
CGCGAAT18650.015.7222661
ACCTAGG35750.015.2383810
ATACCTA38200.014.9831858
TACCTAG39600.014.6275599
CCTATTA23600.013.58726211
CTACACG20150.013.34695512
ATGCGTG25250.013.2468386
TAGGTAC41450.013.226253513
CGCGTAT28100.013.1356781
TCCCTAC24550.013.0613849
CGGATAT22400.012.9361881
GTGGATA43150.012.626364
CCCTACA25050.012.52539510
ACATGCG27100.012.4697764
GGATACC47050.012.45922856
TAAGCGG19100.012.29202332
Produced by FastQC (version 0.11.2)