FastQCFastQC Report
Tue 13 Dec 2016
H2WLHBGX2_n02_63788.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2WLHBGX2_n02_63788.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62617346
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2147270.3429193565629562No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTT99950.0110.486885145
TCGGAAC99400.052.9595145
TCGGAAT122600.037.20093145
AGAGCGG345600.035.12169145
GCGTCGG119700.034.104202145
CGGAAGC376700.031.163403145
TCGGAAA131450.030.228292145
TCGTGTT170600.023.16387145
GATCGGG78000.021.380945145
CGGAAGG186800.020.145782145
GGAAGAC698350.019.457636145
GAGCGTA71950.019.147692145
CGTGTAT134000.018.722546145
AGATCGT290100.017.646175145
ATCGGAG82700.015.78195145
CGCCGTT25750.015.76901145
CGTCGAT14150.015.372993145
CGTATCG9850.014.722697145
GCGTCGT6013850.013.830643145
AGCGTCC94500.013.581105145
Produced by FastQC (version 0.11.2)