FastQCFastQC Report
Tue 13 Dec 2016
H2WLHBGX2_n02_57709.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2WLHBGX2_n02_57709.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62510897
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17460262.793154607907802No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG8152031.3040974280052324No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAGGGGGGGGGGGGGGGGGGGGG5314820.8502229619261422No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1818870.2909684690654815Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGAAAGGGGGGGGGGGGGGGGGGGGGG985670.15767970822751112No Hit
ATATATATATATATATATATATATATATATATATATATATATATATATAT861370.13779517513562475No Hit
GGGGGGGAGAGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG753670.12056617904555106No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGAG1569150.052.2980355
GGGGAGA2040950.051.4231034
GGAGAGG1449850.047.33166
GAGAGGG1433550.046.657387
CGTCGTT59800.043.044495145
AGAGCGG240950.039.321438
GAGAGCG175200.037.237557
AGAGGGG1853700.036.458348
GGGGGAG4286150.036.325063
GAGCGGC139800.035.5714389
GTGGGGA993200.032.3593982
GAGCGGG237200.028.2996319
GGTCGGA61300.026.2611181
TCGGAAC64000.023.905231145
GGGGAAT2215900.023.60640550-54
GTCGGAA61350.023.516782
GAGGGGG4098850.021.7481169
GGGGAGG3257500.021.03624
GAACGGG93600.020.9883339
GGGAATA1836300.020.85996450-54
Produced by FastQC (version 0.11.2)