FastQCFastQC Report
Tue 13 Dec 2016
H2WLHBGX2_n02_57666.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2WLHBGX2_n02_57666.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85285329
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2183850.2560639708618583No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTT101050.072.18626145
CGCCGTT102700.042.14992145
CCGTATA170150.036.904453145
CGTATCG24050.028.943365145
TCGGAAC106250.021.838053145
CGCCGTG113200.021.265938145
TCGTGTT267300.016.601423145
AGAGCGG606000.015.327432145
GTAGATA921200.013.86112145
GCCGTAA138250.013.479098145
CGTGTAT226400.012.202292145
TCGGAAT147750.011.53276145
GTAGATG725950.011.306615145
CGGTGGG644750.011.234843145
GCGTCGG281850.010.856437145
TCGGAAA154950.010.762896145
AAGAGTT1174700.010.246463145
CGGAAGC531450.010.191804145
TCGTGTG150450.08.482295145
TCTCGGG430550.08.437368145
Produced by FastQC (version 0.11.2)