FastQCFastQC Report
Tue 13 Dec 2016
H2WLHBGX2_n02_56590.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2WLHBGX2_n02_56590.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63142148
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8353191.3229182510547473No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG5149250.8155012401541993No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAGGGGGGGGGGGGGGGGGGGGG3041490.48168934639347394No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1137690.18017917287197768Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTT40450.053.955456145
GGGGAGA1574300.035.9904674
GGGGGAG2742100.029.959013
GGGAGAG1495600.029.4008925
GAGAGGG1339250.027.6970067
AGAGGGG1414650.026.4213498
GGAGAGG1469900.025.8264626
TCGGAAC62400.021.148241145
GGGGAAT1511800.019.2775250-54
AGAGCGG244150.018.9729678
GAGGGGG2634350.018.607759
GTGGGGA1028100.017.0796972
GGGAATA1372200.016.27536650-54
CGGAAGC212650.015.650734145
GAGAGCG197100.015.5940287
GGGGGGA8265300.015.4688542
AAAAGGG6839850.015.17837965-69
AAAGGGG6965450.014.79351665-69
AAAAAGG7260950.014.46845865-69
GGAATAA1600800.014.05716550-54
Produced by FastQC (version 0.11.2)