FastQCFastQC Report
Tue 13 Dec 2016
H2WLHBGX2_n02_56052.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2WLHBGX2_n02_56052.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73668310
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4409370.5985436614468284No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAGGGGGGGGGGGGGGGGGGGGG1498010.20334523759266365No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTT98700.059.504982145
TCGGAAC102100.037.283703145
AGAGCGG361800.027.63638145
TCGGAAT140350.025.314486145
CGGAAGC400000.024.272026145
GGGGGAG1880000.021.5285053
GGGGAGA1373200.020.6674694
TCGGAAA153150.018.606342145
AGAGGGG1153100.016.3893018
CGGAAGG233750.015.975007145
GGGAGAG1418150.015.7792835
GAGAGGG1218400.015.4689877
GCGTCGG220750.014.747931145
AGATCGT292300.014.635537145
GGGGGGA4380150.014.1851122
GGAGAGG1391550.013.9763136
GAGGGGG1760700.013.4180439
GGGGAAT1074700.013.33159750-54
TCGTGTT202350.012.899852145
GAGCGTA78150.012.61814145
Produced by FastQC (version 0.11.2)