FastQCFastQC Report
Tue 13 Dec 2016
H2WLHBGX2_n01_63788.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2WLHBGX2_n01_63788.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62617346
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT6759111.0794309295702185TruSeq Adapter, Index 18 (97% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG697920.11145793371696079No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCG59650.044.72826145
GAGCACC548000.034.570324145
TCGGAAC85600.033.455532145
CGGAAGC427850.033.111404145
GTCCGCC106150.031.418282145
CTGAACG120300.029.048655145
TCGGAAT118650.028.597147145
CACACGG220950.028.055416145
AAGAGCC693650.022.649734145
CGGAAGG179450.020.40293145
GATCGGG73500.019.530909145
ACACGTA177650.018.977211145
CGTCTTC1134550.018.1536650-54
GCCGTCT1150900.017.8554550-54
ACGTCCC110500.017.846424145
CCGTCTT1161150.017.74403250-54
TATGCCG1179500.017.4543945-49
TGCCGTC1168750.017.4413250-54
ATGCCGT1183350.017.31183445-49
AGGGGGG1235000.016.56414275-79
Produced by FastQC (version 0.11.2)