FastQCFastQC Report
Tue 13 Dec 2016
H2WLHBGX2_n01_57709.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2WLHBGX2_n01_57709.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62510897
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT720505211.526073606014645TruSeq Adapter, Index 16 (97% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6330191.0126538417773783No Hit
ATATATATATATATATATATATATATATATATATATATATATATATATAT798610.1277553256034704No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA10235450.0109.168259
AGAGCAC10393750.0107.580918
GAAGAGC10815950.0103.456366
AAGAGCA10818800.0103.417857
CGGAAGA10955900.0102.078734
TCGGAAG11226450.099.5771263
GGAAGAG11320050.098.785245
ATCGGAA11476400.097.467132
GATCGGA11647450.095.8223651
GCCGTCT8030250.027.9591650-54
TATGCCG8062350.027.90175445-49
CCGTCTT8055450.027.87169650-54
TCTCGTA7934350.027.78005840-44
CCCGATC7858000.027.75803235-39
ATCTCGT7895400.027.72721340-44
CGTATGC8117000.027.7176545-49
CGTCTTC8107950.027.70946550-54
ATGCCGT8068000.027.64768645-49
CCGATCT7843450.027.62092835-39
TGCCGTC8050800.027.6057650-54
Produced by FastQC (version 0.11.2)