FastQCFastQC Report
Tue 13 Dec 2016
H2WLHBGX2_n01_57666.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2WLHBGX2_n01_57666.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85285329
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT4287030.5026691050227408TruSeq Adapter, Index 15 (97% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1110330.13019003538111462No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGAACG209950.032.909367145
CGTCTTG179100.026.798155145
CACGTCG113650.023.85858145
ATGCCGG548550.021.252611145
GTCTTCG234050.021.218973145
GAGCACC718100.017.30487145
TGCCGTG278800.016.642925145
CGTCTTA204600.014.741109145
CACACGG349250.014.26142145
AGAATCG926800.012.000015145
GTCGTCG57950.011.885367145
TCTCGTT194050.011.4701145
AAGAGCC888550.011.01525145
ACACGTA252400.010.599355145
TGCCGTA121650.09.952835145
CGGAAGC626700.09.382177145
TCGGAAC143400.08.999394145
CGTCGTG40700.08.906725145
CAGAATG1660300.08.3797455145
CCAGTCC864250.08.0365305145
Produced by FastQC (version 0.11.2)