FastQCFastQC Report
Tue 13 Dec 2016
H2WLHBGX2_n01_56590.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2WLHBGX2_n01_56590.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63142148
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT37830885.9913831249453215TruSeq Adapter, Index 14 (97% over 44bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2051780.324946183332249No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA6296550.092.437579
AGAGCAC6417550.090.7579658
CGGAAGA6781700.085.79434
GAAGAGC6832650.085.2647556
AAGAGCA6932050.084.093767
TCGGAAG7055950.082.4854053
ATCGGAA7272600.080.038222
GGAAGAG7476600.077.929745
GATCGGA7494700.077.133481
TATGCCG4281200.027.19954545-49
GCCGTCT4293200.027.04500250-54
CGTCTTC4309800.026.96707550-54
CCGTCTT4312950.026.95544850-54
CGTATGC4320450.026.94707745-49
TCTCGTA4295400.026.93171140-44
ATGCCGT4306500.026.88824845-49
CTCGTAT4300950.026.79716940-44
TATCTCG4283700.026.788340-44
TGCCGTC4304800.026.78182650-54
ATCTCGT4336350.026.66802240-44
Produced by FastQC (version 0.11.2)