FastQCFastQC Report
Tue 13 Dec 2016
H2WLHBGX2_n01_56052.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2WLHBGX2_n01_56052.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73668310
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT18046312.449670692866444TruSeq Adapter, Index 13 (97% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1254770.17032696962913904No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA8056600.034.783349
AGAGCAC8553100.032.7785848
AAGAGCA9442950.029.734877
GAAGAGC9778300.028.6926636
CGGAAGA10574400.026.4688134
GGAAGAG10932650.025.6902715
CGGAAGC433850.025.384153145
TCGGAAG11534550.024.2611853
ATCGGAA12317200.022.7162512
TCGGAAC102000.021.537086145
GAGCACC599400.021.360846145
AGGGGGG2616650.021.02679675-79
GATCGGA13201350.020.9784871
TCGGAAT130550.020.825665145
AAGGGGG2683300.020.58668770-74
CGTCTTC2735200.020.53110750-54
CCGTCTT2738050.020.48749750-54
GCCGTCT2744350.020.43359850-54
TATGCCG2791150.020.11698745-49
ATGCCGT2811150.019.8861845-49
Produced by FastQC (version 0.11.2)