FastQCFastQC Report
Fri 23 Dec 2016
H2WHCBGX2_n01_up_unknown.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2WHCBGX2_n01_up_unknown.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15959609
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGTGGATACCTAGGTACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTTCGGGGAGTTGAAAATAA170020.10653143194172238No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGACAT43850.026.0494171
ACATGCG41800.025.9168824
CGGGACT27600.025.5070781
CATGCGT45100.024.4793625
ATGCGTG46050.024.3492646
GCCAGTA31600.022.8243711
TGCGAGT47600.022.684411
GTGCGAG48350.022.40373210
CGTGAAC25350.021.22994264
TGCGTGC53900.020.7370117
GAGTCGA53100.020.26950814
TTCGATC25900.020.2479652
CGGAATA48700.020.18858368
CTACCCG35150.019.7288976
GTGGATA61700.019.6828024
GGATATC30700.019.666962
AGTCGAC54250.019.64918715
GCGCTAT5150.019.4325751
GGTGGAT63350.019.333713
GGCAACG26900.019.2387352
Produced by FastQC (version 0.11.2)