FastQCFastQC Report
Fri 23 Dec 2016
H2WHCBGX2_n01_up2_unknown.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2WHCBGX2_n01_up2_unknown.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13978154
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182720.13071826222546984No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGACT20750.017.7945351
ACCTAGG47700.017.06781410
TTACGGG11000.016.307848
ACATGCG31150.016.1704674
GGATACC51150.016.053226
TGCGAGT31400.015.49090611
CGCGAAT26500.015.3658681
CGGAATA24300.015.33243268
GTCGCTA29600.015.26855827
ATACCTA58100.014.8439568
TAAGTCG29550.014.7095329
TACCTAG57350.014.6771659
ACATCCG40050.014.64584855
GCCAGTA27850.014.6210241
GTGGATA57050.014.45344
CGCTATG32000.014.44700829
GATACCT58900.013.9395597
ATGCGTG38400.013.9261586
TAGGTAC58500.013.916882513
TATAGGG24850.013.88273260
Produced by FastQC (version 0.11.2)