FastQCFastQC Report
Fri 23 Dec 2016
H2WHCBGX2_n01_50_50_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2WHCBGX2_n01_50_50_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15392063
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT17950.015.5718151
ACCTAGG30850.015.31954210
TTACGGG9600.013.6551218
CTATAAG27700.013.32890346
TACCTAG39600.013.1542169
TAAGTCG15200.012.9364319
ATACCTA40500.012.6915448
TCCGTTA30750.012.34168638
TAGGTAC41300.011.61038313
GTCCTAT32350.011.51768243
CCGTTAA33050.011.37816139
CGTTAAC33200.011.32664440
GTTAACG34200.011.1972841
CCTAGGT43600.011.07702911
TATAAGC32950.010.99580847
CGCGAAT23800.010.5843591
GTCCCTA21300.010.5272988
GCGTATT22700.010.4870412
CGGCTAT18300.010.1826621
TTAGCGT27950.010.1208098
Produced by FastQC (version 0.11.2)