FastQCFastQC Report
Fri 23 Dec 2016
H2WHCBGX2_n01_50_50_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2WHCBGX2_n01_50_50_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18756108
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGGG13150.014.9532018
CGCGAAT26400.013.986671
TAAGTCG23350.013.0011229
AGTTACG25250.012.8441336
CGCGTAT25800.012.5731071
CGCTATG25000.012.55798729
CGCGATA21600.012.1421141
CCTATTA28650.011.92065911
GTCCCTA28300.011.5803658
CGGATAT31950.011.341041
GTCGCTA27400.011.08007327
CGGGACT21850.011.0555681
ACCTAGG31800.010.956717510
GCGTATT31500.010.7337792
GTTCGTA25900.010.65562113
CGACAAT55800.010.5135441
CTATAAG33250.010.48111346
GCGATAG24150.010.4290062
TTCGTAC25950.010.36924120
GCGAATC39750.010.32873252
Produced by FastQC (version 0.11.2)