FastQCFastQC Report
Fri 23 Dec 2016
H2WHCBGX2_n01_50_25_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2WHCBGX2_n01_50_25_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19635633
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAT29700.014.9892391
TTACGGG15050.014.6697158
CGCGATA21600.012.7815211
AGTTACG27400.012.717386
CGGGACT24750.012.4096941
CTCGAAT34800.011.800871
GTCCCTA28400.011.6608738
CTATAAG38650.011.51628646
CGCGTAT23050.011.3786031
TAAGTCG21100.011.2809289
CGCAAAT40100.010.5853941
CCGGGAT72550.010.51238251
GCGATAG25600.010.2425262
CCGGAAC66050.010.188451
CCTATTA32150.010.08632111
TATAAGC43150.010.07554547
CGCTATG28550.010.02959329
ACCTAGG30650.010.0171537
CTGGAAT114750.010.0146971
GCGAATC45050.09.95593552
Produced by FastQC (version 0.11.2)