FastQCFastQC Report
Fri 23 Dec 2016
H2WHCBGX2_n01_50_25_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2WHCBGX2_n01_50_25_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18442151
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGACT20400.012.1795951
CGACAAT58100.011.3445611
CGGGCAT38200.010.8404771
CGGGGAT65150.010.4877241
CGCGAAT26450.010.3069921
CGGAAAC32150.010.3043531
CGCGTAT14900.010.1905341
CGGAAAT30850.010.1792731
CCGGGAT82600.010.1103411
GGAAAAT99800.09.889311
AGTTACG17400.09.7155856
CGGAGAT164150.09.4392171
GCCGAAT27400.09.3199081
TTACGGG11700.09.1401988
CTACACG27400.09.06509312
CGAAAAT39250.09.0558261
GACAATC69800.08.9465312
GGGAAAC58250.08.8863991
CGGACAT57750.08.8438271
ACCTAGG25000.08.83124710
Produced by FastQC (version 0.11.2)