FastQCFastQC Report
Fri 23 Dec 2016
H2WHCBGX2_n01_50_25_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2WHCBGX2_n01_50_25_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16982447
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGGG20400.021.4760468
TAAGTCG36100.020.6408379
CGCGAAT35150.018.8495581
AGTTACG45050.017.4608066
CATGCGT34400.017.3503955
CGCGTAT43900.017.2149141
ATGCGTG34300.017.1999136
ACATGCG33150.017.0680264
GCCAGTA39750.016.755041
CGGGACT25350.016.607611
GTCCCTA42000.016.3449868
CCTATTA42150.016.04157311
GCGTATT48500.015.7922662
TGCGAGT35450.015.66744111
CGCGATA34000.015.3258061
CTATAAG48850.015.04476246
ACCTAGG43400.014.8640277
TACGGGG33150.014.568859
CGGAATA31100.014.42037368
TATAAGC51100.014.38244947
Produced by FastQC (version 0.11.2)