FastQCFastQC Report
Fri 23 Dec 2016
H2WHCBGX2_n01_50_125_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2WHCBGX2_n01_50_125_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18216279
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT21600.012.3018561
CGCGAAT24600.011.6433151
CGACAAT56500.011.2383621
CGGCTAT20300.011.0497131
CGGAAAT30950.010.81545451
CGGGACT22250.010.7016991
TTACGGG10600.010.4143548
GCCGAAT31000.010.2414111
AGTTACG21350.010.1805826
TAAGTCG19500.010.0838439
GCGAATC37750.09.779242
CGGACAT56300.09.7459111
CTACACG30200.09.70952112
CGGAAAC32100.09.5679451
CGGAGAT157450.09.556031
CGCAAAT33450.09.2849611
GGAAAAT101100.09.2160811
GACAATC66400.09.1969432
CCCTACA36750.09.10537510
ACCTAGG26550.09.09529910
Produced by FastQC (version 0.11.2)