FastQCFastQC Report
Fri 23 Dec 2016
H2WHCBGX2_n01_50_125_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2WHCBGX2_n01_50_125_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13999124
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAT20450.013.1622581
CGGGACT15700.011.8692471
CGGGCAT30300.011.3890221
CGACAAT45600.011.1245281
CTACACG22000.010.50632712
TTACGGG8300.010.3908948
CGGAAAC23600.010.3818661
GGAAAAT78550.010.3240661
ATACCTA22650.010.052338
ACCTAGG19750.09.95642510
TCCCTAC25800.09.627259
CGCGTAT15800.09.6100281
CGGGGAT49350.09.5799341
CCGGGAT63050.09.5781581
CTCGAAT21950.09.4329131
GCGAATC29300.09.4200952
CGCAAAT27150.09.4056971
CGGGTAT9950.09.3641811
GTCCCTA18200.09.2878618
CCCGTAT9350.09.2269351
Produced by FastQC (version 0.11.2)