FastQCFastQC Report
Fri 23 Dec 2016
H2WHCBGX2_n01_50_0625_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2WHCBGX2_n01_50_0625_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17448763
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT14850.011.6195041
CGGCAAT42950.011.0881731
CGCGAAT22100.010.9307461
CGGGGAT55300.010.5464331
GGAAAAT103900.010.4958011
CGGAAAC31800.010.3095691
CCGGGAT68800.010.2827541
CGGGCAT41450.010.2405821
CCCGGAT38250.09.5635361
CGCCTAT14500.09.519981
GCCGAAT30650.09.1200791
GTCGAAT33700.09.0114951
CGGAAAT31200.08.9593091
GGGAAAT61200.08.8530371
CACGAAT46400.08.7762321
CGGAGAT136350.08.7571951
CCCGAAT21300.08.7489961
GCGGAAT26550.08.708721
GTCCCTA23200.08.6243688
CGGGAAT38300.08.46981
Produced by FastQC (version 0.11.2)