FastQCFastQC Report
Fri 23 Dec 2016
H2WHCBGX2_n01_50_0625_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2WHCBGX2_n01_50_0625_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18499178
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAT23250.014.1003791
CGGGACT22950.012.6306791
CGCGTAT26550.012.6077421
GTCCCTA25450.012.0637098
AGTTACG23200.011.3018646
CGCGATA18350.011.0954791
CGGATAT31450.010.7531461
TAAGTCG23550.010.6932149
CGGGGAT54750.010.5259781
ACCTAGG31750.010.43055310
CGGAAAC34900.010.2834311
CCGGGAT69150.010.1305721
TTACGGG12650.010.089978
CGGCAAT48400.010.0531891
CCTATTA29650.010.0058411
CGGGCAT38750.09.9741631
CTATAAG32750.09.90361746
CGGACAT51950.09.8976221
CGGGTAT13300.09.8596641
GGAAAAT100850.09.8205581
Produced by FastQC (version 0.11.2)