FastQCFastQC Report
Fri 23 Dec 2016
H2WHCBGX2_n01_50_0625_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2WHCBGX2_n01_50_0625_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20925090
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCAT48450.012.3217831
CGCGAAT24700.011.7355531
CGGAAAT36800.011.15887451
CGGGGAT64150.010.91995051
GGAAAAT130550.010.0180591
CGCGTAT18050.09.9413961
CGGGACT25900.09.8594631
CGACAAT70000.09.8101651
CGGACAT58300.09.7072571
CTACACG34600.09.67116512
CGGAAAC41250.09.4531331
CGGGAAT42250.09.3927421
CGGAGAT166200.09.1980111
CCCTACA40600.09.17651610
GCCGAAT37350.09.1467291
CCGGGAT82050.09.1264611
CGGATAC70750.09.0720981
CGGGTAT17950.08.8433061
CCGGAAT48750.08.777451
CCCGAAT24400.08.6270291
Produced by FastQC (version 0.11.2)