FastQCFastQC Report
Fri 23 Dec 2016
H2WHCBGX2_n01_100_50_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2WHCBGX2_n01_100_50_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17122339
Sequences flagged as poor quality0
Sequence length75
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGACT22350.016.5211471
CGGAATA19050.016.4793368
ACCTAGG34900.014.5308617
CGGATAT33050.014.304841
TTACGGG12900.014.1735298
CGCGATA23500.013.5099551
GTCCCTA20950.013.5027338
CGCGAAT27850.013.2584421
ACTCACG23750.013.2180463
GTGGATA39900.013.1463331
ACATGCG29650.012.7999064
ATACCTA39800.012.7429665
CTATAAG27350.012.48966846
TACCTAG40950.012.4692836
CGGCTAT17500.012.4232841
TAGGTAC42500.012.25680610
ATGCGTG31350.012.2157226
TAAGTCG27800.012.037059
CGGAAAC28550.011.7246411
CGCGTAT31200.011.7242541
Produced by FastQC (version 0.11.2)