FastQCFastQC Report
Fri 23 Dec 2016
H2WHCBGX2_n01_100_50_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2WHCBGX2_n01_100_50_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15592109
Sequences flagged as poor quality0
Sequence length75
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTAGG26500.012.3671527
CGGGCAT35900.011.8233111
CGCGTAT16650.011.6065251
CCGGGAT82950.011.0660771
CGGCTAT18250.010.9671451
CGACAAT44600.010.9096981
ATACCTA31950.010.6901245
CGGGTAT11650.010.663631
GGAAAAT71600.010.4586391
GCCGAAT24850.010.4150981
CGGGACT18700.010.3341531
GTGGATA36100.010.323931
CGGGGAT58250.010.0712051
CCTAGGT34750.010.0266488
TACCTAG35800.010.0216889
CGGAAAC23450.010.0067841
CCCGAAT12500.09.9385031
AGTTACG13250.09.8943876
GTCCCTA14850.09.7569058
CGGAGAT166950.09.7562761
Produced by FastQC (version 0.11.2)