FastQCFastQC Report
Fri 23 Dec 2016
H2WHCBGX2_n01_100_50_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2WHCBGX2_n01_100_50_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13647237
Sequences flagged as poor quality0
Sequence length75
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT16350.025.95442644
TCGTATG18100.023.63710245
ACGTCTG30400.018.61076215
TATGCCG23100.018.52213548
TTACGGG11400.016.946268
AGTCACG25200.016.83892428
TATCTCG26800.015.70505941
CACGAGT28150.015.31994731
TAAGTCG22400.014.6306859
GTCACGA29900.014.30743829
CACGTCT41800.014.11276314
AGTTACG24350.013.74393756
CGGAATA15650.013.66693768
ACCTAGG34350.013.65839410
ACGAGTG33250.013.38545932
GTCCCTA19950.013.31491858
CGCGTAT25800.013.2428421
GTATGCC36250.013.23076647
CGCGAAT20100.013.2208811
CGCTATG22000.013.015676529
Produced by FastQC (version 0.11.2)