FastQCFastQC Report
Fri 23 Dec 2016
H2WHCBGX2_n01_100_25_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2WHCBGX2_n01_100_25_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14434873
Sequences flagged as poor quality0
Sequence length75
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGGG8550.016.1391758
TAAGTCG17300.013.9583769
CGCGAAT20800.013.2724941
ACCTAGG27250.012.9137
CGGGACT20700.012.3363661
CGGAAAC25200.012.3244591
GTCCCTA17750.012.2441788
AGTTACG21450.012.0636046
CGGAATA16150.011.9620668
TACCTAG30400.011.6897926
TATAGGG13300.011.67248660
CGCGATA15300.011.5028281
CGCGTAT22250.011.4769791
CCGGGAT62050.011.4008691
ATACCTA30950.011.2591055
GTCGCTA21150.011.25539627
TACACGA21250.011.20153613
GTTCGTA17150.011.063333513
GTGGATA36700.011.0013421
CTACACG20700.010.99927412
Produced by FastQC (version 0.11.2)