FastQCFastQC Report
Fri 23 Dec 2016
H2WHCBGX2_n01_100_25_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2WHCBGX2_n01_100_25_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15486458
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTAGG30600.012.1756147
ATACCTA34000.011.0594468
GGGAAAT47100.010.7702711
TACCTAG36350.010.345536
GCCGAAT26600.010.119131
CCGGGAT67950.010.0555521
CCTAGGT39600.09.8439418
CGGGACT17900.09.8321251
CGCGAAT21850.09.791981
CGACAAT50950.09.617771
GGAAAAT84700.09.6152111
CGGGCAT31450.09.5461631
GTGGATA45050.09.3453441
GGCGAAT17400.09.321351
CGGCAAT44850.09.3100741
TAGGTAC39400.09.28107413
TTACGGG9300.09.2734818
CGGGGAT54550.09.17283251
CTAGGTA43050.09.1351589
AGTTACG15000.08.9702396
Produced by FastQC (version 0.11.2)