FastQCFastQC Report
Fri 23 Dec 2016
H2WHCBGX2_n01_100_25_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2WHCBGX2_n01_100_25_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12969423
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGGG9350.018.8163538
AGTTACG17700.013.8389186
TCGTATG19950.013.83574945
CTCGTAT20400.013.69909944
CGGGTAT9650.013.2312851
CGCGAAT20100.013.0480361
CGGGACT16450.012.796511
TACGGGG14450.011.9366029
TATGCCG24300.011.92747948
ACCTAGG29750.011.82760410
CGGATAT21450.011.2615541
CGGGCAT28900.011.22425651
ATACCTA33100.010.9430448
TACCTAG32900.010.9047579
TAGGTAC34400.010.32933613
TATCTCG27150.010.16577641
CTCGAAT21150.010.1160021
GTCACGT28950.010.01050929
CGTATGC30700.010.00270146
GTGGATA37600.09.8202721
Produced by FastQC (version 0.11.2)