FastQCFastQC Report
Fri 23 Dec 2016
H2WHCBGX2_n01_100_125_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2WHCBGX2_n01_100_125_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13625804
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAT17550.013.3713941
CCGGGAT61300.011.4282691
CGCGTAT14800.011.19243051
CGCAAAT23550.010.84390451
CGGAGAT125650.010.8212791
CGACAAT40500.010.5660241
CGGGCAT31050.010.3363271
GCGAATC26550.09.8761742
GGAAAAT69550.09.8741741
CGGAAAC23150.09.8387031
ACCTAGG24350.09.7753177
CGGAAAT25600.09.70593551
GGCGAAT16750.09.6834021
GACAATC50400.09.5837872
GGGACAT27100.09.5507361
CTCGAAT23100.09.4118171
CGGATAT20650.09.3586421
TTACGGG8851.8189894E-129.3550368
ATACCTA26450.09.2611725
CGGGACT17250.09.2026651
Produced by FastQC (version 0.11.2)