FastQCFastQC Report
Fri 23 Dec 2016
H2WHCBGX2_n01_100_125_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2WHCBGX2_n01_100_125_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18057848
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGGG13700.017.1230668
TAAGTCG26300.016.134029
CGCGTAT30500.015.3873761
ACCTAGG40500.015.1623127
CGGGACT25250.014.0767151
CGCGAAT28750.013.8033831
AGTTACG32500.013.8008216
CGCGATA22350.013.5872221
TACCTAG45350.013.2378326
GTGGATA49400.013.0629171
CGGAATA23150.012.96452668
GCGTATT35300.012.6082252
CATGCGT32050.012.4873785
TAGGTAC49300.012.38570610
ATACCTA48150.012.1813085
GCGATAG24000.012.0755512
GTCCCTA29250.011.9121118
CGGACAT59550.011.7635881
CGATAGT25250.011.7509663
ACATGCG34300.011.5675524
Produced by FastQC (version 0.11.2)