FastQCFastQC Report
Fri 23 Dec 2016
H2WHCBGX2_n01_100_125_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2WHCBGX2_n01_100_125_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16757466
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGGG15550.019.3004768
TAAGTCG26850.017.601689
GTCCCTA25100.016.6299448
CATGCGT34700.016.2062555
CTATAAG33450.015.988749546
TGCGAGT33550.015.62910411
ATGCGTG38250.015.3334796
CCTATTA28400.015.18359811
CGCGTAT32150.015.1341691
ACATGCG36350.014.9011034
ACCTAGG38400.014.7330210
AGTTACG33950.014.5318026
CGCGATA25900.014.5226931
TATAAGC37150.014.30366447
CGGGACT21650.014.0263671
GTCCTAT38900.013.83535943
GTCGCTA30200.013.7083327
CGCGAAT26350.013.6198751
CGGAATA23000.013.34901168
GCGTATT37550.013.2305652
Produced by FastQC (version 0.11.2)