FastQCFastQC Report
Fri 23 Dec 2016
H2WHCBGX2_n01_100_0625_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2WHCBGX2_n01_100_0625_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14048669
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGGG9150.018.0974278
TAAGTCG16950.014.6539559
CGCGTAT18650.014.2475951
AGTTACG19150.013.1525846
CGCGAAT19450.012.774461
ACCTAGG26250.011.8280467
CGCGATA16250.011.4675351
GCGTATT22300.011.2945472
ATGCGTG26900.011.1589666
TAAGCGG17450.011.07078720
ATAAGCG17800.011.04686919
CATGCGT24900.010.9467385
CGGGACT21000.010.8456171
CGGAAAC26550.010.7880571
GTCGCTA20800.010.78092427
CGGATAT23800.010.7296231
ATACCTA31050.010.4453565
CGCTATG20750.010.30823229
CGGGGAT47650.010.1389921
CCGGGAT53500.010.1268781
Produced by FastQC (version 0.11.2)