FastQCFastQC Report
Fri 23 Dec 2016
H2WHCBGX2_n01_100_0625_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2WHCBGX2_n01_100_0625_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14389169
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGGG11700.019.4598648
CGGGACT18200.018.012891
TAAGTCG27700.016.937239
CGCGTAT31700.016.3291341
CGCGAAT27150.016.0151821
CTATAAG32850.015.755346
GTCCCTA25000.015.4546718
ACATGCG29650.015.1266474
ATGCGTG31250.014.9041686
TAAGCGG25250.014.89225520
ACCTAGG33650.014.86509510
TATAAGC35950.014.78087647
ATAAGCG26100.014.53928319
TGCGAGT30850.014.31334211
CGCTATG28800.014.01568629
GTTCGTA27950.013.94697113
TACCTAG37350.013.8542779
CCTATTA28500.013.79895611
GTCGCTA30700.013.70996527
TTCGTAC28300.013.65296420
Produced by FastQC (version 0.11.2)