FastQCFastQC Report
Fri 23 Dec 2016
H2WHCBGX2_n01_100_0625_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2WHCBGX2_n01_100_0625_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18214119
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA335620.1842636473386388No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAT42450.019.7530671
GCGAATC55650.015.3129992
CGCTATG21800.013.92671429
CGCGTAT21150.012.8891031
TAAGTCG17750.012.8269669
TTACGGG10500.012.4845448
ACCTAGG35500.012.43830510
CGAATCC81250.012.1440623
TACCTAG41250.011.7916229
TATAAGC29700.011.73399447
TAGGTAC37200.011.68445213
GTTCGTA19200.011.67865713
CTGTCGT62850.011.08811960
GTCGCTA28350.010.83065327
CGCGATA15550.010.65164851
AGTTACG22050.010.639516
CGGGGAT51950.010.62772751
AACGCGG65600.010.57096636
CCGGGAT67250.010.4675211
AATCCTC90950.010.3936755
Produced by FastQC (version 0.11.2)